phragmitis

Neocamarosporium Crous & M.J. Wingf., Persoonia 32: 273 (2014).

Type species:
Neocamarosporium goegapense P.W. Crous & M.J. Wingfield, Persoonia 32: 273 (2014).

Marine species:
Neocamarosporium phragmitis D.N. Wanasinghe, E.B.G. Jones & K.D. Hyde, Mycosphere 9(2): 306 (2018).

MycoBank: MB554240                                                                                             Facesoffungi: FoF04099

Saprobic on culms of Phragmites australis. Sexual morph Ascomata 150−200 μm high, 120−180 μm diameter (x ̅ = 180.6 × 147.6 µm, n = 10), black, semi-immersed, confluent, gregarious, cupulate when dry, globose, uniloculate, with an apapillate ostiole. Ostiole central, short, slightly sunken, minute and inconspicuous at the surface, smooth, ostiolar canal filled with brown to hyaline cells. Peridium 10–25 µm wide at the base, 30–40 µm wide in sides, comprising 4–5 layers of reddish-brown to brown, cells, lighter and flattened towards the inside, of thin-walled cells of textura angularis. Hamathecium comprising numerous, 2–3 µm (n = 40) wide, filamentous, branched, septate, pseudoparaphyses. Asci 60–90 × 14–17 µm (x ̅ = 75.9 × 15.4 µm, n = 40), 8-spored, bitunicate, fissitunicate, cylindrical-clavate to cylindrical, short pedicellate (8–12 µm long), apex rounded with a minute ocular chamber. Ascospores 12–16 × 6–8 µm (x ̅ = 14.9 × 7.2 µm, n = 50), overlapping biseriate, muriform, mostly ellipsoidal, with 3 transverse septa and one longitudinal septum, deeply constricted at the middle septum, slightly constricted at remaining septa, initially hyaline, becoming pale brown at maturity, upper part wider than lower part, slightly paler, rounded at both ends, conical at the lower end. Asexual morph: undetermined.
The description was based on Hyde et al. (2018).

Key to marine species in the genus:

1a. Occurring on salt marsh plants                                                         2
1b. On other substrates and sediments                                                   3
2a. Ascospore 12–16 × 6–8 µm, hyaline to pale brown muriform
host Phragmites australis                                                                      N. phragmitis
2b. Conidia 8–12 × 4–6 μm, dark brown, muriform
host Salicornia sp.                                                                                N. salicorniicola
2c. Conidia 9–11.5 x 3.5–5 μm, pale yellow to brownish, 1–septate
host Halimonie sp.                                                                                N. obiones
3a. On tortoise shell                                                                              N. chersinae
3b. Isolated from sediment from saline lakes                                           4
4a. Conidia (9–)11–12(−13) × 4–7(−8) μm, muriform, globose to
obovoid to ellipsoid, golden brown                                                         N. jorjanensis
4b. Conidia (8–)12(−16) × (2–)2.5(−4) μm, muriform, variable from
globose to obovoid to ellipsoid, golden brown                                         N. persepolisi
4c. Conidia (8–)12(−16) × (2–)2.5(−4) μm, ellipsoid to
tear–drop–shaped hyaline                                                                    N. solicola

Key references:

Hyde KD, Chaiwan N, Norphanphoun C, Boonmee S, Camporesi E, Chethana KWT, Dayarathne MC, de Silva NI, Dissanayake AJ, Ekanayaka AH, Hongsanan S, Huang SK, Jayasiri SC, Jayawardena RS, Jiang HB, Karunarathna A, Lin CG Liu JK, Liu NG, Lu YZ, Luo ZL, Maharachchimbura SSN, Manawasinghe IS, Pem D, Perera RH, Phukhamsakda C, Samarakoon MC, Senwanna C, Shang QJ, Tennakoon DS, Thambugala KM, Tibpromma S, Wanasinghe DN, Xiao YP, Yang J, Zeng XY, Zhang JF, Zhang SN, Bulgakov TS, Bhat DJ, Cheewangkoon R, Goh TK, Jones EBG, Kang JC, Jeewon R, Liu ZY, Lumyong S, Kuo CH, McKenzie EHC, Wen TC, Yan JY, Zhao Q (2018). Mycosphere notes 169–224. Mycosphere 9(2): 271-430.

Crous PW, Shivas RG, Quaedvlieg W, van der Bank M, Zhang Y, Summerell BA, Guarro J, Wingfield MJ, Wood AR, Alfenas AC, Braun U, Cano-Lira JF, García D, Marin-Felix Y, Alvarado P, Andrade JP, Armengol J, Assefa A, den Breeÿen A, Camele I, Cheewangkoon R, De Souza JT, Duong TA, Esteve-Raventós F, Fournier J, Frisullo S, García-Jiménez J, Gardiennet A, Gené J, Hernández-Restrepo M, Hirooka Y, Hospenthal DR, King A, Lechat C, Lombard L, Mang SM, Marbach PAS, Marincowitz S, Marin-Felix Y, Montaño-Mata NJ, Moreno G, Perez CA, Pérez Sierra AM, Robertson JL, Roux J, Rubio E, Schumacher RK, Stchigel AM, Sutton DA, Tan YP, Thompson EH, van der Linde E, Walker AK, Walker DM, Wickes BL, Wong PTW, Groenewald JZ (2014). Fungal Planet Description Sheets: 214–280. Persoonia 32:184-306.

Wanasinghe DN, Hyde KD, Jeewon R, Crous PW, Wijayawardene NN, Jones EBG, Bhat DJ, Phillips AJL, Groenewald JZ, Dayarathne MC, Phukhamsakda C, Thambugala KM, Bulgakov TS, Camporesi E, Gafforov Y, Mortimer PE, Karunarathna SC (2017I. Phylogenetic revision of Camarosporium (Pleosporinae, Dothideomycetes) and allied genera. Stud Mycol 87:207-256.

Papizadeh M, Wijayawardene NN, Amoozegar MA, Saba F, Shahzadeh Fazeli SA, Hyde KD (2017). Neocamarosporium jorjanensis, N. persepolisi, and N. solicola spp. nov. (Neocamarosporiaceae, Pleosporales) isolated from saline lakes of Iran indicate the possible halotolerant nature for the genus. Mycol Prog 17(5): 661-679.

Image:

Neocamarosporium phragmitis (MFLU 17-0585, holotype). a, b Ascomata on host substrate. c Section of ascoma. d Pseudoparaphyses. e–g Asci. h–j Ascospores. Scale bars: a = 1 mm, b = 200 µm, c = 100 µm, d = 5 µm, e–g = 20 µm, h–i = 10 µm.
The figure was taken from Hyde et al. (2018).

• 
  Fig 1