Genus

Dipodascus

Species

tetrasporeus

Author

Nagah. & Abdel-Wahab, International Journal of Systematic and Evolutionary Microbiology 58 (4): 1040-1046 (2008)

Class

Saccharomycetes, Subclass Saccharomycetidae

Order

Saccharomycetales

Family

Dipodascaceae

Synonymy:

Dipodascus Lagerh., Jahrbücher für Wissenschaftliche Botanik 24: 549 (189

Type species:

Dipodascus albidus Lagerh., Jahrbücher für Wissenschaftliche Botanik 24: 549-565 (1892)

Marine species:

Dipodascus tetrasporeus Nagah. & Abdel-Wahab, International Journal of Systematic and Evolutionary Microbiology 58 (4): 1040-1046 (2008)

Index Fungorum Number: 533142                         Faceoffungi Number: N/A

After 10 days on MEYE agar at 20°C, colonies are 25–30 mm in diameter, white, flat, dry, centrally powdery and swelling, with finely hairy margins. Hyphae are 3–5 µm wide, with rounded apices, and with some basitonous branching, with slight differentiation between main and lateral branches, branches soon disarticulating into cylindrical arthroconidia (2.5–4.0-5.0–36.0 µm). Abundant true mycelia and arthroconidia are formed. On ME agar medium, hyphae and arthroconidia produce clusters of globose to subglobose blastospores, 4.0–6.0 µm in diameter. Gametangia are located on opposite sides of septa or on separate hyphae. Asci are subspherical to broadly ellipsoidal, 5–10 µm wide and 6–14 µm long, and contain one to four ascospores. Ascospores are globose to subglobose 3.0–5.0 µm in diameter, with an irregular exosporium and gelatinous sheath. The species is homothallic. D-glucose and galactose are fermented but sucrose, maltose, lactose, raffinose and melibiose are not. The following carbon compounds are assimilated: D-glucose, galactose, L-sorbose, D-xylose, D-arabinose (weak), D-ribose (weak), ethanol, glycerol, glucono- b-lactone, DL-lactic acid, succinic acid and D-galacturonic acid. No growth occurs on sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, L-arabinose, L-rhamnose, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, methyl a-D-glucoside, salicin, 2-ketogluconic acid, 5-ketogluconic acid, citric acid, inositol or D-glucuronic acid. The nitrogen compounds ethylamine, lysine and cadaverine are assimilated but potassium nitrate and sodium nitrite are not. Growth occurs at 27°C and is weak at 30°C but does not occur at all at 33°C on MEYE agar. Growth occurs on vitamin-free medium. No growth occurs on 50% glucose/yeast extract agar. Growth occurs in the presence of 100 p.p.m. cycloheximide. No growth occurs in the presence of 10% sodium chloride. No starch-like substances are produced. The diazonium blue B reaction is negative. Urease activity is negative. The G+C content of the nuclear DNA is 40.7 mol% (by HPLC).

Description based on Nagahama et al. (2008).

 

Key references:

Nagahama T, Abdel–Wahab MA, Nogi Y, Miyazaki M, Uematsu K, Hamamoto M, Horikoshi K (200). Dipodascus tetrasporeus sp. nov., an ascosporogenous yeast isolated from deep–sea sediments in the Japan Trench. International Journal of Systematic and Evolutionary Microbiology. 58(4):1040–1046.

 

Type & Location:
Other Specimens:
Substratum:
Was isolated from sediments collected from the deep–sea floor in the Japan Trench, Pacific Ocean.
Habitat:
Distribution:
Japan
Pertinent Literature:
Description based on Nagahama et al. (2008).
Comments:
NOTES: Currently some 20 Dipodascus species are known, but only D. tetrasporeus has been reported from the marine environment which was isolated and described from sediments collected from the deep–sea floor in the Japan Trench, Pacific Ocean. The phylogenetic position of this species is not fully resolved. In the clade comprising the genera Dipodascus, Galactomyces and Geotrichum, this is the only species that forms asci that bear four ascospores. The ascospore is surrounded by an irregular exosporium wall, similar to what is observed in the genus Galactomyces, but they are released by rupture, which is characteristic of Dipodascus and not Galactomyces.

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